Some genomic features of Enterococcus durans isolated from fermented milk products

Some genomic features of Enterococcus durans isolated from fermented milk products


Liubov B. Zelena

Enterococci are widely used in the production of various fermented food products and can be applied as probiotics. However enterococci are typical opportunistic pathogens that cause nosocomial infections, they are involved in distribution of antibiotic resistance and process of food spoilage. In the present study genome of 9 Enterococcus durans strains isolated from fermented milk products was analyzed in order to study intraspecies variability and identify pathogenicity, antibiotic resistance genes as well as genes encoding some enterocins. A high level of intraspecies polymorphisms was revealed as the result of amplification with primer to tetranucleotide repeat: each sample had unique amplicon pattern. On the contrary, no detectable differences between samples were observed using M13 primer which contains minisatellite repeat. E.durans strains were also genetically characterized to identify genes coding enterocins A, B, L50B and P. Among all only enterocin P gene was revealed in all samples. To assess the pathogenicity of isolated E. durans strains PCR with primers to virulence determinants, gelatinase gelE, cytolysin cylA, hyaluronidase hyl, adhesion factors asa1 and esp, was performed. None of these was detected in strains analyzed. PCR screening for antibiotic resistance genes (aph, ant, tetM, vanA, vanB) showed that all strains had vancomycin and tetracycline resistance genes and 20% of strains possessed aminoglycoside resistance gene. Thus, the results obtained indicate that accurate molecular-genetic analysis of dairy products is required to prevent harmful effects on human health.



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